TRIS Hydrochloride, Ultra Pure
CAS No: 1185-53-1
Catalog No.: T1185UP
Purity: ≥99%
| Size | Price |
|---|---|
| 100G | $30.00 |
| 500G | $50.00 |
| 1KG | $75.00 |
| 5KG | $350.00 |
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Technical Information
Tris(hydroxymethyl)aminomethane hydrochloride, commonly known as Tris Hydrochloride, has been primarily used for simplifying the process of preparing Tris buffer solutions. The use of solid Tris Hydrochloride avoids using more hazardous hydrochloric acid, and allows a more precise ratio to be measured to achieve a specific buffer pH. This also avoids any possibility of titration overshoot, where too much acid is added requiring additional Tris base, resulting in a different ionic strength than desired.
A Tris/Tris Hydrochloride Buffer table is referenced to show specific buffer quantities required for a wide range of pH buffers.
CAS Number 1185-53-1
Name TRIS Hydrochloride
Synonyms TRIS Hydrochloride, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride
Molecular Formula (HOCH2)3C-NH2<• HCl
Molecular Weight 157.60
Specifications
| Test | T1185UP, Ultra Pure |
|---|---|
| Purity (by titration, on dried basis) | NLT 99.0% |
| Absorbance (1M solution in water) | |
| 260nm | NMT 0.05 |
| 280nm | NMT 0.05 |
| Identification (By IR) | IR matches with known reference |
| Loss On Drying (110°C) | NMT 0.4% |
| Melting Point | 150°C to 153°C |
| pH (1M solution in H2O) | 3.7 to 4.7 |
| pKa (at 20°C) | 8.0 to 8.4 |
| Moisture (by KFT) | NMT 0.5% |
| Residue on ignition | NMT 0.1% |
| Solubility (1M solution in water) | Clear, complete and colorless |
Material is of non-animal, non-human origin
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Resources
TRIS HCl Base Mixing Table
| pH at 5°C | pH at 25°C | pH at 37°C | Grams/liter for 0.05 M Tris Base | Grams/liter for 0.05 M Tris HCl |
|---|---|---|---|---|
| 7.55 | 7.00 | 6.70 | 0.47 | 7.28 |
| 7.66 | 7.10 | 6.80 | 0.57 | 7.13 |
| 7.76 | 7.20 | 6.91 | 0.67 | 7.02 |
| 7.89 | 7.30 | 7.02 | 0.80 | 6.85 |
| 7.97 | 7.40 | 7.12 | 0.97 | 6.61 |
| 8.07 | 7.50 | 7.22 | 1.18 | 6.35 |
| 8.18 | 7.60 | 7.30 | 1.39 | 6.06 |
| 8.26 | 7.70 | 7.40 | 1.66 | 5.72 |
| 8.37 | 7.80 | 7.52 | 1.97 | 5.32 |
| 8.48 | 7.90 | 7.62 | 2.30 | 4.88 |
| 8.58 | 8.00 | 7.71 | 2.65 | 4.44 |
| 8.68 | 8.10 | 7.80 | 2.97 | 4.02 |
| 8.78 | 8.20 | 7.91 | 3.34 | 3.54 |
| 8.88 | 8.30 | 8.01 | 3.70 | 3.07 |
| 8.98 | 8.40 | 8.10 | 4.03 | 2.64 |
| 9.09 | 8.50 | 8.22 | 4.36 | 2.21 |
| 9.18 | 8.60 | 8.31 | 4.65 | 1.83 |
| 9.28 | 8.70 | 8.42 | 4.90 | 1.50 |
| 9.36 | 8.80 | 8.51 | 5.13 | 1.23 |
| 9.47 | 8.90 | 8.62 | 5.32 | 0.96 |
| 9.56 | 9.00 | 8.70 | 5.47 | 0.76 |
| 9.67 | 9.10 | 8.79 | 5.53 | 0.69 |
