Technical Information

Tris(hydroxymethyl)aminomethane, commonly known as “Tris” or “Tris Base” has been used as a buffer in a wide variety of applications, including biological systems. Tris buffer solutions at a wide range of pH are generated using Tris Base and hydrochloric acid, or alternatively with Tris Base and Tris Hydrochloride in a defined ratio and concentration. The use of solid Tris Hydrochloride avoids using more hazardous hydrochloric acid, and allows a more precise ratio to be measured to achieve a specific buffer pH.

Tris Base is also used as a primary base standard due to its purity, stability and high equivalent weight. It can be dried at a relatively high temperature (100°C) and is not hygroscopic 1.

CAS Number 77-86-1

Name TRIS

Synonyms Tris (Hydroxymethyl) Aminomethane, addex-tham, tromethanmin,

tromethane, tris(hydroxymethyl)methylamine, tromethamine, Addex-Tham, Aminotrimethylolmethane,

Aminotris(hydroxymethyl)methane, 1,1,1-tris(hydroxymethyl)-methanamine, Pehanorm,

Talatrol, Tham-E, Trimethylolaminomethane, Tris, Tris amino, Tris buffer,

Molecular Formula (HOCH2)3C-NH2

Molecular Weight 121.14