HEPES Bio Pure

HEPES, Bio Pure

CAS No: 7365-45-9

Catalog No.: H7365BPG

Purity: ≥99.5%

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100G $40.00
250G $85.00
500G $125.00
1KG $195.00

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Technical Information

HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It's effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.

1 N.E. Good, et al., Biochemistry, 5, 467 (1966).

CAS Number 7365-45-9

Name HEPES Ultra Pure Grade

Synonyms 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid

Molecular Formula C8H18N2O4S

Molecular Weight 238.30


Specifications

Test H7365UP, Ultra Pure
Appearance White crysatlline powder
Assay (by titration, on dried basis) NLT 99.5%
Solubility (1M solution H2O) Clear, complete and colorless
Identification (by IR) Conforms to structure
Melting Point --
Water (105°C for 3 hours) ≤1.0%
Water (KF) ≤0.1%
pH (5% solution in water) 5.0 - 6.5
pKa (@25°C) 7.45 - 7.65
UV Absorption (1M @290nm) ≤0.04
UV Absorption (1M @280nm) ≤0.05
UV Absorption (1M @260nm) ≤0.05
Residue on Ignition ≤0.1%
Insoluble Matter --
Heavy Metals (Lead) ≤0.0005%

Biological Impurity Testing

Test H7365UP, Ultra Pure
Endotoxin NMT 0.1 EU/mg
Endotoxin (0.2% solution in H2O ≤ 0.10 EU/mL
Bioburden NMT 100 CFU/g
RNase, DNase, Nickase, Protease None detected
Cell culture test Pass

Trace Impurity Testing

Test H7365UP, Ultra Pure
Aluminum (Al) --
Arsenic (As) --
Barium (Ba) --
Bismuth (Bi) --
Cadmium (Cd) --
Calcium (Ca) --
Chloride (Cl) --
Chromium (Cr) --
Cobalt (Co) --
Copper (Cu) --
Iron (Fe) ≤0.0005%
Lithium (Li) --
Magnesium (Mg) --
Manganese (Mn) --
Molybdenium (Mo) --
Nickel (Ni) --
Potassium (K) --
Sodium (Na) --
Strontium (Sr) --
Zinc (Zn) --
Sulfate (S04) ≤0.05%

Material is of non-animal, non-human origin


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References

1 N.E. Good, et al., Biochemistry, 5, 467 (1966).

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