HEPES, Bio Pure

CAS No: 7365-45-9

Catalog No.: H7365BPG

Purity: ≥99.5%

100G $40.00
250G $85.00
500G $125.00
1KG $195.00


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HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It's effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.

1 N.E. Good, et al., Biochemistry, 5, 467 (1966).

CAS Number 7365-45-9
Name HEPES Ultra Pure Grade
Synonyms 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
Molecular Formula C8H18N2O4S
Molecular Weight 238.30

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TestH7365UP, Ultra Pure
Appearance White crysatlline powder
Assay (by titration, on dried basis) NLT 99.5%
Solubility (1M solution H2O)  Clear, complete and colorless
Identification (by IR) Conforms to structure
Melting Point --
Water (105°C for 3 hours) ≤1.0%
Water (KF) ≤0.5%
pH (5% solution in water) 5.0 - 6.5
pKa (@25°C) 7.45 - 7.65
UV Absorption (1M @290nm) ≤0.04
UV Absorption (1M @280nm) ≤0.05
UV Absorption (1M @260nm) ≤0.05
Residue on Ignition ≤0.1%
 Insoluble Matter --
Heavy Metals (Lead)  ≤0.0005%
Biological Impurity Testing
Test H7365UP, Ultra Pure
Endotoxin NMT 0.1 EU/mg
Endotoxin (0.2% solution in H2O ≤ 0.10 EU/mL
Bioburden  NMT 100 CFU/g
RNase, DNase, Nickase, Protease  None detected 
Cell culture test  Pass
Trace Impurity Testing  
 Test  H7365UP, Ultra Pure
Aluminum (Al)  --
Arsenic (As) -- 
Barium (Ba)  -- 
Bismuth (Bi)  -- 
Cadmium (Cd)  -- 
Calcium (Ca) --
Chloride (Cl) --
Chromium (Cr) --
Cobalt (Co) --
Copper (Cu) --
Iron (fe) ≤0.0005%
Lithium (Li) --
Magnesium (Mg) --
Manganese (Mn) --
Molybdenium (Mo) --
Nickel (Ni) --
Potassium (K) --
Sodium (Na) --
Strontium (Sr) --
Zinc (Zn) --
Sulfate (S04) ≤0.05%

Material is of non-animal, non-human origin.


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