TRIS, Ultra Pure, Molecular Biology Grade

CAS No: 77-86-1

Catalog No.: T7786A

Purity: ≥99%

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100G $25.00
500G $35.00
1KG $50.00
5KG $225.00
10KG $395.00

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TECHNICAL INFORMATION

Tris(hydroxymethyl)aminomethane, commonly known as “Tris” or “Tris Base” has been used as a buffer in a wide variety of applications, including biological systems. Tris buffer solutions at a wide range of pH are generated using Tris Base and hydrochloric acid, or alternatively with Tris Base and Tris Hydrochloride in a defined ratio and concentration. The use of solid Tris Hydrochloride avoids using more hazardous hydrochloric acid, and allows a more precise ratio to be measured to achieve a specific buffer pH.

Tris Base is also used as a primary base standard due to its purity, stability and high equivalent weight. It can be dried at a relatively high temperature (100°C) and is not hygroscopic 1.

CAS Number 77-86-1
Name TRIS
Synonyms Tris (Hydroxymethyl) Aminomethane, addex-tham, tromethanmin,
tromethane, tris(hydroxymethyl)methylamine, tromethamine, Addex-Tham, Aminotrimethylolmethane,
Aminotris(hydroxymethyl)methane, 1,1,1-tris(hydroxymethyl)-methanamine, Pehanorm,
Talatrol, Tham-E, Trimethylolaminomethane, Tris, Tris amino, Tris buffer,
Molecular Formula (HOCH2)3C-NH2
Molecular Weight 121.14

Related Products

TRIS Hydrochloride


Specification

TestT7786A, Ultra Pure
Appearance White crysatlline powder
Purity (by titration, on dried basis) NLT 99.0%
Solubility (1% solution in H2O) Clear, complete and colorless
Identification (by IR) Conforms to structure
Moisture ≤ 0.2%
Insoluble Matter ≤ 0.005%
pH (1M solution in water) 10.5 to 11.5
Melting Point 168.0 to 172° C
UV Absorption (40% in H20 @290nm) ≤ 0.05
Heavy Metals as Lead ≤ 1ppm
Iron (Fe) ≤ 1ppm
DNase None detected
RNase None detected
Protease None detected

Material is of non-animal, non-human origin.


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RESOURCES

TRIS BASE MIXING TABLE

pH at 5°CpH at 25°CpH at 37°CGrams/liter 
for 0.05 M 
Tris HCl
Grams/liter
for 0.05 M
Tris Base
7.55 7.00 6.70 7.28 0.47
7.66 7.10 6.80 7.13 0.57
7.76 7.20 6.91 7.02 0.67
7.89 7.30 7.02 6.85 0.80
7.97 7.40 7.12 6.61 0.97
8.07 7.50 7.22 6.35 1.18
8.18 7.60 7.30 6.06 1.39
8.26 7.70 7.40 5.72 1.66
8.37 7.80 7.52 5.32 1.97
8.48 7.90 7.62 4.88 2.30
8.58 8.00 7.71 4.44 2.65
8.68 8.10 7.80 4.02 2.97
8.78 8.20 7.91 3.54 3.34
8.88 8.30 8.01 3.07 3.70
8.98 8.40 8.10 2.64 4.03
9.09 8.50 8.22 2.21 4.36
9.18 8.60 8.31 1.83 4.65
9.28 8.70 8.42 1.50 4.90
9.36 8.80 8.51 1.23 5.13
9.47 8.90 8.62 0.96 5.32
9..56 9.00 8.70 0.76 5.47
9.67 9.10 8.79 0.69 5.53

REFERENCES

1. (J. Fossum, P. Markunas and J. Riddick, Analytical Chemistry, 1951, 23, 491)